„PLANT BREEDING AND SEED PRODUCTION“, Vol. 12, No. 1-2 (2006)

 

 

Dimitrijević М., Petrović Sofija, Belić M.

MODELS FOR GENOTYPE BY ENVIRONMENT INTERACTION ESTIMATION ON HALOMORPHIC SOIL [Abstract]

 

Орлюк А.П., Жужа В.А.

ОЦЕНКА КАЧЕСТВА ЗЕРНА ОЗИМОЙ МЯГКОЙ ПШЕНИЦЫ НА РАННИХ ЭТАПАХ СЕЛЕКЦИИ [Abstract]

 

Zdravković Jasmina, Stanković Ljiljana, Todorović Vesna, Pavlović N., Zdravković M.

SEED YIELD OF LETTUCE IN DIFFERENT SAWING PERIODS [Abstract]

 

Dimitrijević M., Petrović Sofija, Belić M., Vuković Nataša

PHENOTYPIC VARIATION OF SPIKE PARAMETERS IN WHEAT GROWN ON AMELIORATED SOIL [Abstract]

 

Panayotov N.

QUALITY OF VEGETABLE SEEDS: MAIN FACTORS AND MODERN ASPECTS [Abstract]

 

Brdar Milka, Kraljević-Balalić Marija, Maksimović Ivana, Kobiljski B.

BORON TOLERANCE IN NS WHEAT LINES [Abstract]

 

Dodig D., Stanković S., Milicević-Nikodijević Slađana, Jović Miroslava

NEWLY REGISTERED SMALL GRAINS CULTIVARS FROM ZAJEČAR [Abstract]

 

Petrović Sofija, Dimitrijević M., Belić M.

PHENOTYPIC VARIABILITY OF YIFXD COMPONENTS IN WHEAT GROWN ON DIFERENT SOIL TYPES [Abstract]

 

Žilić Slađana, Srebrić Mirjana, Hadži-Tašković Šukalović Vesna, Anđelković S.

BIOCHEMICAL CHARACTERIZATION OF ZP SOYBEAN GENOTYPES [Abstract]

 

Djurić Veselinka, Malešević M., Panković L.

THE INFLUENCE OF CULTTVAR, YEAR AND NITROGEN SUPLLY ON QUALITY PARAMETERS OF BREAD WHEAT (Triticum aestivum L) [Abstract]

 

Petrović Sofija, Dimitrijević M., Belić M.

THE EFFECT OF MELIORATION OF HALOMORPHIC SOIL ON SPIKE TRAITS IN WHEAT [Abstract]

 

Putnik Delić Marina, Jerković Z.

PHYTOPATHOLOGIC CONSEQUENCES OF PSEUDO RESISTANCE TO Puccinia triticina [Abstract]

 

Antonova Galina, Nikolova Vesselina, Petkova Valent1na, Tomlekova Nasya

CHEMICAL MUTAGENESIS IN HEAD CABBAGE Brassica oleracea var. capitata L.

I. SURVIVAL AND VARIABILITY OF PROPERTIES FROM MORPHOLOGICAL

CHARACTERISTIC OF M1 GENERATION [Abstract]

 

Nikolova Vesselina, Antonova Galina, Tomlekova Nasia, Petkova Valentina

CHEMICAL MUTAGENESIS IN HEAD CABBAGE (Brassica oleracea var. capitata L.). III EFFECT OF ETHYL METHANE SULPHONATE ON MYCOSPOROGENESIS AND POLLEN FERTILITY [Abstract]

 

Tomlekova Nasya, Galina Antonova, Vesselina Nikolova, Valentina Petkova

CHEMICAL MUTAGENESIS IN HEAD CABBAGE (Brassica oleracea var capitata L.).

IV. DNA POLYMORPHISM [Abstract]

 

Červenskij., Gvozdenović D., Gvozdanović-varga Jelica, Nikolić Zorica, Balaž F.

SURVEY OF CABBAGE EXPERIMENTAL HYBRIDS (Brassica oleracea var. capitata L.) [Abstract]

 



 

Published in „Plant breeding and Seed Production“ Vol. 12, No. 1-2  (2006), pp. 7-14
© 2006 Serbian Association of Plant Breeders & Seed Producers
S. Bajica 1, Belgrade - Zemun, 11185

Serbia

 

UDC: 575.22:631.445

Original scientific paper

 

 

MODELS FOR GENOTYPE BY ENVIRONMENT INTERACTION ESTIMATION ON HALOMORPHIC SOIL

 

DIMITRIJEVIĆ M., PETROVIĆ SOFIJA, BELIĆ M.

 

 

SUMMARY

 

In genotype by environment interaction estimation, as well as,.in total trial variability analysis several models are in use. The most oftenly used are Analysis of variance, Eberhart and Russell model and AMMI model. Each of the models has its own specificities, in the way of sources of varia­tion comprehension and treatment. It is known that agriculturaly less productive environments increase errors; diminish reaction differences between genotypes and decrease repeatability of conditions during years. A sample consisting on six bread wheat varieties was studied in three veg­etation periods on halomorphic soil, solonetz type in Banat (vil. Kumane). Genotype by environ­ment interaction was quantified using ANOVA, Eberhart and Russell model and AMMI model. The results were compared not only on pure solonetz soil (control), but also on two level of ameliora­tion (25 and 50t/ha phosphor-gypsum).

 

 

Corresponding author: Prof. Dr. MIODRAG DIMITRIJEVIĆ, mishad@polj.ns.ac.yu

 

 

Return to content



 

Published in „Plant breeding and Seed Production“ Vol. 12, No. 1-2  (2006), pp. 15-21
© 2006 Serbian Association of Plant Breeders & Seed Producers
S. Bajica 1, Belgrade - Zemun, 11185

Serbia

 

 

UDC: 631.52:633.11

Original scientific paper

 

 

ОЦЕНКА КАЧЕСТВА ЗЕРНА ОЗИМОЙ МЯГКОЙ ПШЕНИЦЫ НА РАННИХ ЭТАПАХ СЕЛЕКЦИИ

 

ОРЛЮК А.П., ЖУЖА В.А.

 

 

SUMMARY

 

В расщепляющихся поколениях гибридов мягкой озимой пшеницы наибольшийудельный вес занимаю комбинации с положительным доминированием и промежуточным наследованием содержания белка и клейковины, качества клейковины, и сидементации. Показатель неследуемости баиболее высокий у признака „седиментация“, он в меньшей степени, чем другие признаки, подвергается генотип-средовых взаимодействиям в градиенте изменчивых агроэкологических факторов и является одмин из наиболее надежных маркерных признаков в селекции на повышение содержания белка и клейковины силы муки.

 

 

Corresponding author: Профессор, доктор биологических наук ОРЛЮК АНАТОЛИЙ, Институт земледелия южного региона УААН, Херсон, Украина.

 

 

Return to content



 

Published in „Plant breeding and Seed Production“ Vol. 12, No. 1-2  (2006), pp. 23-26
© 2006 Serbian Association of Plant Breeders & Seed Producers
S. Bajica 1, Belgrade - Zemun, 11185

Serbia

 

 

UDC: 635.52:631.53

Original scientific paper

 

 

SEED YIELD OF LETTUCE IN DIFFERENT SAWING PERIODS

 

ZDRAVKOVIĆ JASMINA, STANKOVIĆ LJILJANA, TODOROVIĆ VESNA, PAVLOVIĆ N., ZDRAVKOVIĆ M.

 

 

SUMARRY

 

This paper is a result of researching the number of lettuce (Lactuca saliva L.) seed per plant in two sawing periods. The presumption was that the traits: total number of flowers and total num­ber of flowers per head, are highly correlated and that they represent the components of seed yield. In the second sawing period the skipping of the heading phase was provoked. Skipping of the heading phase is the effect of the long day, when plants transfer to flowering phase from phase of 4-6 permanent leaves. This research aimed to investigate the decrease of the seed number de­pending on sowing time lettuce seed crop.

Ten lettuce varieties (Nansen, Majska kraljica, Safir, Universal, Ravel, Atraction, Superble, Type-5140, Ljubljanska ledenka and Brauner) for total flower number and the number of flowers per flower head in two sawing periods were compared. Two-factorial trial in random block system with four replications was performed. The correlation of the two researched traits was medium (r = 0,706) without significance. Regression coefficient showed the correlation of these traits. Vari­ance analysis showed that variance of genotype was not significant: the other factor, sowing time and interaction (genotype x sowing time) were not significant. The coefficient of phenotype vari­ance was higher than the genetic variance coefficient for both traits.

 

 

Corresponding author: Dr. ZDRAVKOVIĆ JASMINA, Institut za istraživanja u poljoprivredi "Srbija" Beograd, Centar za povrtarstvo Smederevska Palanka, Karađorđeva 71, 11240 Smederevska Palanka

 

 

Return to content



 

Published in „Plant breeding and Seed Production“ Vol. 12, No. 1-2  (2006), pp. 27-33
© 2006 Serbian Association of Plant Breeders & Seed Producers
S. Bajica 1, Belgrade - Zemun, 11185

Serbia

 

 

UDC: 631.41:633.11

Original scientific paper

 

 

PHENOTYPIC VARIATION OF SPIKE PARAMETERS IN WHEAT GROWN ON AMELIORATED SOIL

 

DIMITRIJEVIĆ M., PETROVIĆ SOFIJA, BELIĆ M., VUKOVIĆ NATAŠA

 

 

SUMMARY

 

Less productive agricultural soil, that is solonetz type, could be ameliorated in order to en­hance usage value and broaden agricultural land. Grain weight per spike and spike weight of wheat grown on solonetz soil were examined in the article. Phenotypic variation of these traits as a response to environmental condition in six bread wheat varieties was followed through genotype by environment interaction in three vegetation periods. The results were compared on pure solonetz (control) and ameliorated solonetz (25 and 50t/ha phosphor-gypsum) to determine ef­fect of amelioration on these two yield components.

 

 

Corresponding author: Prof. Dr. Miodrag Dimitrijević, mishad@polj.ns.ac.yu

 

Return to content



 

Published in „Plant breeding and Seed Production“ Vol. 12, No. 1-2  (2006), pp. 35-44
© 2006 Serbian Association of Plant Breeders & Seed Producers
S. Bajica 1, Belgrade - Zemun, 11185

Serbia

 

 

UDC: 635.1

Review paper

 

 

QUALITY OF VEGETABLE SEEDS: MAIN FACTORS AND MODERN ASPECTS

 

PANAYOTOVN.1

 

 

SUMMARY

 

One of the most precise methods for qualification of vegetable seeds, which gives more full information, than just values of germination, is the determination of seed vigour.

In the present paper are described the history of development, definitions, principles and priorities of the seed vigor and its significance for agricultural practices and especially for com­plex characterization of vegetable seed quality. The main part of the presentation is dedicated to the importance of seed vigor. Included are the other non-destructive and rapid methods for es­tablishment of the vegetable seed quality and their relation with vigor. Some of the suitable and available indexes and parameters of vegetable seed for estimation of the current status and for prevention of the losses are pointed out. In this article are presented the results of last years inves­tigations form which concern the different means for establishing the quality and viability sta­tus of vegetable seeds.

 

Key words: vegetable seeds, quality, viability, vigor test, germination

 

 

Corresponding author:            Dr. NIKOLAY PANAYOTOV, Agricultural University, 12 Mendeleev Str., Plovdiv, Bulgaria. E-mail: nikpan@au-plovdiv.bg

 

 

Return to content



 

Published in „Plant breeding and Seed Production“ Vol. 12, No. 1-2  (2006), pp. 45-48
© 2006 Serbian Association of Plant Breeders & Seed Producers
S. Bajica 1, Belgrade - Zemun, 11185

Serbia

 

 

UDC: 631.41:633.11

Original scientific paper

 

 

BORON TOLERANCE IN NS WHEAT LINES

 

BRDAR MILKA, KRALJEVIĆ-BALALIĆ MARIJA, MAKSIMOVIĆ IVANA, KOBILJSKI B.

 

 

SUMMARY

 

Boron is an essential micronutrient for higher plants. Present in excessive amounts boron becomes toxic and can limit plant growth and yield. Suppression of root growth is one of the symptoms of boron toxicity in wheat. This study was undertaken to investigate the response of 10 perspective NS lines of wheat to high concentrations of boron. Analysis of root growth was done on young plants, germinated and grown in the presence of different concentrations of boric acid (0, 50, 100 and 150mg/l). Significant differences occurred between analyzed genotypes and treat­ments regarding root length. Average suppression of root growth was between 11.6 and 34.2%, for line NS 252/02 are even noted 61.4% longer roots at treatments in relation to the control. Lines with mean suppression of root growth less than 20% (NS 101/02, NS 138/01, NS 53/03 and NS 73/02) may be considered as boron tolerant. Spearman’s coefficients showed high level of agree­ment regarding rang of root length for genotypes treated with 100 and 150 mg H3BO3/1.

 

Key words: wheat, boron toxicity, roots

 

 

Corresponding author: Milka Brdar, milkabrdar@yahoo.com

 

Return to content



 

Published in „Plant breeding and Seed Production“ Vol. 12, No. 1-2  (2006), pp. 49-54
© 2006 Serbian Association of Plant Breeders & Seed Producers
S. Bajica 1, Belgrade - Zemun, 11185

Serbia

 

 

UDC: 633.1

Original scientific paper

 

NEWLY REGISTERED SMALL GRAINS CULTIVARS FROM ZAJECAR

 

DODIG D., STANKOV1C S., MILICEVIC-NIKODiJEVIC SLADANA, JOVIC MIROSIAVA

 

 

SUMMARY

 

The paper presents principal properties of 5 newcultivars of small grains created in the Cen­tre for Agricultural and Technological Research in Zaječar. They are as follows: Arena (winter bread wheat), Premium (winter malting barley), Tango (winter triticale), Centar (spring oat) and Balsa (spring naked barley). In 2004 and 2005 these cultivars were registered by the Ministry of Ag­riculture, Forestry and Water Management of the Republic of Serbia. The Arena cultivar has good indices of technological quality and high yield genetic potential which is successfully preserved even in dry years. The Premium cultivar, besides high yield capacity, has a very low protein grain content what makes it especially suitable for brewers industry. Triticale Tango is intended, in the first place, for animal feeds and like Arena cultivar, is highly tolerant to draught. Spring oat Centar is Zajecars first made oat cultivar in this group of small grains. Cultivar Balsa is the first spring six-row naked barley in Serbia and Montenegro. Creating of Balsa cultivar is the attempt to meet the future demands on the market. By the act of registering above mentioned cultivars the Centre in Zaječar has completed and enlarged its already existing variety of small grains assortment.

 

Key words: cultivar, small grains, Center-Zaječar, yield, quality

 

 

Corresponding author: Dr. DEJAN DODIG, IZIUP SRBIJA, Centar za poljoprivredna i tehnološka istraživanja, Zaječar

 

 

Return to content



 

Published in „Plant breeding and Seed Production“ Vol. 12, No. 1-2  (2006), pp. 55-59
© 2006 Serbian Association of Plant Breeders & Seed Producers
S. Bajica 1, Belgrade - Zemun, 11185

Serbia

 

 

UDC: 631.41:633.1

Original scientific paper

 

 

PHENOTYPIC VARIABILITY OF YIFXD COMPONENTS IN WHEAT GROWN ON DIFERENT SOIL TYPES

 

PETROVIĆ SOFIJA, DIMITRIJEVIĆ M., BELIĆ M.

 

 

SUMMARY

 

The vast share of agricultural soil in Vojvodina province is quality chernozemic soil. How­ever, there is still a share of halomorphic soil, solonetz type, that are not convenient for agricul­tural production. Phenotypic variability and correlation of spike parameters in wheat varieties de­rived in Novi Sad are given in this article. Wheat genotypes were sown on chernozemic soil, as well as, on solonetz soil (controll and two levels of melioration 25 and 50t/ha phosphor-gypsum). The goal was to investigate varietal differences in reaction to melioration and soil type. The results ob­tained reveal the highest mean values of examined yield components on chernozem, as well as, different genotype reaction to meliorative measures on solonetz type of soil.

 

Key words: wheat, spike parameters, chernozem, solonetz

 

 

Corresponding author: Dr. SOFIJA PETROVIĆ, vanredni profesor, Poljoprivredni fakultet, Novi Sad. sonjap@polj.ns.ac.yu  

 

 

Return to content



 

Published in „Plant breeding and Seed Production“ Vol. 12, No. 1-2  (2006), pp. 61-66
© 2006 Serbian Association of Plant Breeders & Seed Producers
S. Bajica 1, Belgrade - Zemun, 11185

Serbia

 

 

UDC: 577:635.655

Original scientific paper

 

BIOCHEMICAL CHARACTERIZATION OF ZP SOYBEAN GENOTYPES

 

ŽILIĆ SLAĐANA, SREBRIĆ MIRJANA, HADŽI-TAŠKOVIĆ ŠUKALOVIĆ VESNA, ANĐELOVIĆ S.

 

 

SUMMARY

 

In order to biochemically characterise ZP soya bean genotypes the analyses of the main chemical composition (contents of ash, fibre, total proteins, oil), contents of protein fraction and trypsin inhibitor, as well as, the activity of enzymes urease, lipoxygenase 1 (LOX-1) andperoxidase (POD) in grain of five ZP soya bean cultivars (Lana, Lidija, Bosa, Nena and ZPS 015) were per­formed. As a check and in order to compare results, the analyses of grain of cultivars not derived at the Maize Research Institute, Zemun Polje, were also carried out.

According to the protein content, the cultivar Nena stands out among ZP soya bean geno­types. The content of the grain total protein and water soluble proteins amounted to 43.34% and 34.97%, respectively. The cultivar Lana was also one of the best analysed ZP genotypes. A genotype with recessive genes for the Kunitz trypsin inhibitor was derived by conventional selection meth­ods and therefore, grain of this cultivar has an extremely low content of the total trypsin inhibitor amounting, on the average, to 14. mg g-1. It is interesting that the highest, i.e. lowest LOX-1 activi­ties were detected in the varieties Lana and Nena, respectively. According to the POD activity, the analysed soya bean cultivars can be classified into the following three groups: cultivars with high activity (EpEp genotypes), cultivars with low activity (epep genotypes), while the cultivar Bosa, heterozygous for this trait, belongs to the third group (Epep).

 

Key words: Biochemical characterization, soybean variety

 

 

Corresponding author: dr SLAĐANA ŽILIĆ, szilic@mrizp.co.yu

 

 

Return to content



 

Published in „Plant breeding and Seed Production“ Vol. 12, No. 1-2  (2006), pp. 67-71
© 2006 Serbian Association of Plant Breeders & Seed Producers
S. Bajica 1, Belgrade - Zemun, 11185

Serbia

 

 

UDC: 633.11:631.84

Original scientific paper

 

THE INFLUENCE OF CULTTVAR, YEAR AND NITROGEN SUPLLY ON QUALITY PARAMETERS OF BREAD WHEAT (Triticum aestivum. L)

 

DJURIĆ VESELINKA, MALEŠEVIĆ M., PANKOVIĆ L.

 

 

SUMMARY

 

Field experiments with 3 winter wheat (Triticum aestivum L); Lasta, Sremica and Pobeda was applied nitrogen (rate N) as follows: 0, 60, 120 and 180 kgNha'1 from 2000 to 2002.

The varieties differed in their biological and production characteristics as well as in techno­logical quality. The analyzed samples belonged to the international ISDV (Internationale Internationale Stickstoff Dauer Versuche) stationary field trial established at the Rimski Sancevi Experiment Field of the Institute of Field and Vegetable Crops in Novi Sad. Improvement of end use quality in winter wheat depends on thorough understanding of the influences of environ­ment, variety, and their interaction.Grain protein content (GPC), sedimentation value (SED), en­ergy dough, Hagberg falling number (HFN) and bread crumb quality number were measured. Highly significant differences were detected among the environments (A), rate N (B) and varieties (C) for each of the quality variables. Both variety (V) and environment (E) had a significant effect on quality traits. Significant V x E interactions indicated that quality trait evaluations must be un­dertaken for environments. The most influence on protein content and sedeimentation value have been climatitic condition. According to lot of environment influence on falling number and dow energy the main part of variance it is genotype and phenotype variability.

 

Key words: winter wheat, protein content, quality bread, nitrogen

 

 

Corresponding author: Mr GORAN JEVTIĆ, krmnobilje@ptt.yu 

 

Return to content



 

Published in „Plant breeding and Seed Production“ Vol. 12, No. 1-2  (2006), pp. 73-77
© 2006 Serbian Association of Plant Breeders & Seed Producers
S. Bajica 1, Belgrade - Zemun, 11185

Serbia

 

 

UDC: 631.41:633.11

Original scientific paper

 

 

THE EFFECT OF MELIORATION OF HALOMORPHIC SOIL ON SPIKE TRAITS IN WHEAT

 

PETROVIC, SOFIJA, DIMITRIJEVIC, M., BELIC, M.

 

 

SUMMARY

 

The wheat is agricultural cultivar that requires suitable soil in respect to fertility, physical characteristics and chemical reaction. Halomorphic soil, solonetz type, could be utilized for wheat growing by using ameliorative measures, only.

The effect of solonetz amelioration using phosphor-gypsum in amount of 25 and 50 t/ha on the number of grains and grain weight per spike of different wheat varieties was studied in the arti­cle. According to the results, wheat varieties exhibit notable variability in reaction to soil ameliora­tion. The research should be in continuation in order to select suitable wheat genotypes for putt­ing less productive soil in use.

 

Key words: wheat, number of grains and grain weight per spike, solonetz

 

 

Corresponding author:            dr SOFIJA PETROVIĆ, vanredni profesor, Poljoprivredni fakultet, Novi Sad. sonjap@polj.ns.ac.yu 

 

 

Return to content



 

Published in „Plant breeding and Seed Production“ Vol. 12, No. 1-2  (2006), pp. 79-82
© 2006 Serbian Association of Plant Breeders & Seed Producers
S. Bajica 1, Belgrade - Zemun, 11185

Serbia

 

 

UDC: 633.11:632.4

Original scientific paper

 

 

PHYTOPATHOLOGIC CONSEQUENCES OF PSEUDO RESISTANCE TO Puccinia triticina

 

PUTNIK DELIĆ MARINA, JERKOVIĆ Z.

 

 

SUMMARY

 

Winter wheat genotypes were tested in greenhouse in spring according to Puccinia triticina resistance characters of seedling with and without second leafs. In the field conditions, leaf senescence as Puccinia triticina and facultative parasites development were estimated. The one of the aims of this paper was to establish, which combinations or single expression of the resis­tance characters to Puccinia triticina at seedling stage separated the resistant reaction from pseudo resistance. Latency period was the character of the resistance which divided to parasite specific from wide stress reaction expressed throw quick senescence of down leafs and abundant growth of the first upper one. The reaction of seedlings to obligate parasite on pseudo resistant could be expressed throw lower reaction type. Value of mentioned character was higher on the va­riety Slavia when second leafs were removed after incubation. Senescence of below leafs was in di­rection of facultative and saprophyte fungi development (toxin producers or transporters) in semi arid region with longer continual periods of moisture in spring like during 2005. The first appear­ance of scab in field was on genotypes with relative severe simultaneous attack of Pyrenophora tritici repentis and Septoria tritici. They were pseudo resistant to Puccinia triticina.

 

Key words: Puccinia triticina, leaf senescence, facultative parasites

 

 

Corresponding author: Prof. dr NOVO PRŽULJ, przulj@ifvcns.ns.ac.yu 

 

 

Return to content



 

Published in „Plant breeding and Seed Production“ Vol. 12, No. 1-2  (2006), pp. 83-87
© 2006 Serbian Association of Plant Breeders & Seed Producers
S. Bajica 1, Belgrade - Zemun, 11185

Serbia

 

 

UDC: 575.4:633.42

Original scientific paper

 

 

CHEMICAL MUTAGENESIS IN HEAD CABBAGE Brassica oleracea var. capitata L.

I. SURVIVAL AND VARIABILITY OF PROPERTIES FROM MORPHOLOGICAL

CHARACTERISTIC OF M1 GENERATION

 

ANTONOVA GALINA, NIKOLOVA VESSELINA, PETKOVA VALENT1NA, TOMLEKOVA NASYA

 

 

SUMMARY

 

The purpose of study was to establish the chemical mutagene influence - ethyl methane sulphonate (EMS) on the germination and the survival of head cabbage plants in M1 generation, variety Ditmarsko, as well as to establish the phenotype expression changes of properties from morphological characteristic in economic maturity.

It was established that concentration increasing of chemical mutagen ethyl inethan sulphonate by head cabbage variety Ditmarsko seed treatment leads to decreasing of germinated plants as that correlation does not regard the survival (fig. 1). The number of survived plants ob­tained by seeds treated with a higher concentration of ethyl methane sulphonate is bigger than the tested variant with the lowest EMS concentration. The chemical mutagen ethyl methane sulphonate applied in concentration of 0.5%, 0.6% and 0.7% does not cause entire lethality in head cabbage M1 germination Ditmarsko variety seeds

The chemical mutagen causes inhibiting or stimulating influence on property expressions from morphological characteristic of M1 genotypes (tabl. 1) that together with a higher variability of a prevail part of properties (tabl. 2) determines the presence of big phenotype diversity regard­ing to studied properties even in the first mutant generation.

 

Key words: head cabbage; chemical mutagenesis; morphological characteristic; germination; survival

 

 

Return to content



 

Published in „Plant breeding and Seed Production“ Vol. 12, No. 1-2  (2006), pp. 89-93
© 2006 Serbian Association of Plant Breeders & Seed Producers
S. Bajica 1, Belgrade - Zemun, 11185

Serbia

 

 

UDC: 575.4:633.42

Original scientific paper

 

 

CHEMICAL MUTAGENESIS IN HEAD CABBAGE (Brassica oleracea var. capitata L.).

III EFFECT OF ETHYL METHANE SULPHONATE ON MYCOSPOROGENESIS AND POLLEN FERTILITY

 

NIKOLOVA VESSELINA, ANTONOVA GALINA, TOMLEKOVA NAS1A, PETKOVA VALENTINA

 

 

SUMMARY

 

The aim of present investigation was to study the effect of different ethyl methane sulfonate (EMS) concentrations (0.5, 0.6 and 0.7%) on microsporogenesis and on the expression of pollen fertility in M1 plants, obtained from treated cabbage seeds (variety "Ditmarsko"). The investiga­tion was conducted to identify particular stages of male sterility expression at cytological level.

In treatment of seeds from Brassica oleracea var. capitata L., variety "Ditmarsko" with EMS (concentration 0.5, 0.6 and 0.7%) completely sterile plants were not obtained. The increase of EMS concentration reflected in decrease of pollen fertility and in grow of the percentage of plants with low values of this character (Table 1). The cytological investigation indicated, that the geno­type influenced on the chromosome behavior during mikrosporogenesisin in plants, received from treated with EMS mutagen seeds (Table 2 and 3).

The disturbances during different meiotic stages (early stages - diakinesis and metaphase one in plant № 18 (Fig. 1) and later phases - second metaphase - anaphase in plant № 36) could be a reason for breakdown of significant part of microspores (89.9 and 91.0% sterile pollen grains in plants № 36 and 18) (Fig.3).

 

Key words:     microsporogenesis, pollen fertility and sterility, ethyl methane sulfonate, Brassica olerasea var. capitata L.

 

 

Corresponding author: Dr. Miodrag Dimitrijević, mishad@polj.ns.ac.yu

 

Return to content

 



 

Published in „Plant breeding and Seed Production“ Vol. 12, No. 1-2  (2006), pp. 95-100
© 2006 Serbian Association of Plant Breeders & Seed Producers
S. Bajica 1, Belgrade - Zemun, 11185

Serbia

 

 

UDC: 575.4:633.42

Original scientific paper

 

 

CHEMICAL MUTAGENESIS IN HEAD CABBAGE (Brassica oleracea var capitata L.).

IV. DNA POLYMORPHISM

 

TOMLEKOVA NASYA, GALINA ANTONOVA, VESSELINA NIKOLOVA, VALENTINA PETKOVA

 

 

SUMMARY

 

Inter Simple Sequence Repeats (ISSR) method was applied for a detection of induced van ability on a DNA level in M1 generation after Ethylmethan Sulphonate (EMS) chemical treatment in concentrations 0.5,0.6 and 0.7% of seeds from head cabbage, Brassica oleraceae L., var. Capitata, cv. Ditmarsko.

Different patterns of mutant plants obtained from the studied species were established by randomly selected primer sequences among tandem repeats. Polymorphisms were revealed among M1 and control plants. Diverse DNA patterns of mutant plants were identified. As a result of the pre-breeding screening done in M1, altered plants were selected and seeds for growing the next M2 generation were gathered.

The sequenced primers selected in the NCBI gene database were applied for amplification reactions in cabbage successfully (Table 1, Table 2). The DNA patterns obtained after amplification by ISSR technique were reproducible. The application of the method to study mutant populations in M1 was approved. Polymorphism among mutants and wild type plants was established (Figure 1, Figure 2). In some cases ISSRs (reaction with primer i 1) were able to distinguish variability in individ­ual plants of the same variety (Figure 1).

 

Key words:     head cabbage, Brassica oleraceae L, ISSR-PCR, induced mutagenesis, EMS, DNA polymorphism

 

 

Corresponding author:            dr TOMLEKOVA NASYA, Maritsa Vegetable Crops Research Institute, 4003, Plovdiv, Bulgaria.

E-mail: tomlekovi@mail.bg,  nasia.tomlekova@gmail.com

 

Return to content



 

Published in „Plant breeding and Seed Production“ Vol. 12, No. 1-2  (2006), pp. 101-105
© 2006 Serbian Association of Plant Breeders & Seed Producers
S. Bajica 1, Belgrade - Zemun, 11185

Serbia

 

 

UDC: 633.42

Original scientific paper

 

 

SURVEY OF CABBAGE EXPERIMENTAL HYBRIDS (Brassica oleracea var. capitata L.)

 

ČERVENSKIJ., GVOZDENOVIĆ D., GVOZDANOVIĆ-VARGA JELICA, NIKOLIĆ ZORICA, BALAŽ F.

 

 

SUMMARY

 

Cabbage takes up significant area in vegetable sowing structure, and one of the factors of im­proving this production is adequate varieties selection. During the breeding process, experimen­tal hybrids are tested in relation to currently grown varieties and hybrids in production. In this pa­per the characteristics of 18 cabbage genotypes are presented, out ofwhich there are 9 experimen­tal hybrids, 4 varieties and 5 hybrids from broader production. Cabbage genotypes in the trial are of differing lenght of growing season, as well as differing head weight. Properties variability analy­sis was performed using PCA method, where two main components were chosen based on scree test, and these were used to define 57.7%. Head weight and head diameter are properties based on which the tested hybrids were graded into quantitatively different groups.

 

Key words: experimental hybrids, grouping, cabbage, PCA analysis.

 

 

Return to content